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This supports the competition that these pretreatments have an affect on the protein construction and substrate natural environment, As a result lowering hydrolysis over when a single pretreatment is utilised.

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The information acquired through the time program hydrolysis of Hoki and Gemfish roe homogenates (Figure one) demonstrate progressive hydrolysis by the 3 proteases used in the study as revealed by the rise in L-serine equivalent contents in excess of the hydrolysis time. For Hoki roe, Alcalase exhibited an Over-all larger DH compared to HT and FP-II, and the hydrolysis with FP-II was reduce than that of HT. These benefits are in agreement With all the results of Ahmmed et al. [thirteen] who reported Alcalase to have a far better hydrolysis potential with Hoki roe homogenate than HT and FP-II, beneath the very same hydrolysis situations, and which the DH is increased with growing the protease concentration.

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